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Image Search Results
Journal: Tuberculosis (Edinburgh, Scotland)
Article Title: Differential inflammasome activation signatures following intracellular infection of human macrophages with Mycobacterium bovis BCG or Trypanosoma cruzi
doi: 10.1016/j.tube.2016.09.026
Figure Lengend Snippet: Human THP-1 cells were differentiated into macrophages by activation with PMA (50 ng/ml), rested, and exposed to LPS (100 ng/ml) or infected with M. bovis BCG for 3 and 18 h. Transcripts were quantified using the RT Profiler Human Inflammasome PCR Array (SA Biosciences/Qiagen) and triplicate samples. Data generated was analyzed using the web-based PCR Array Data Analysis software for threshold cycle (Ct) value determinations and contrast analysis was performed using LimmaCt in the HT-qPCR software package to identify genes that were differentially expressed.
Article Snippet: Data generated was analyzed using the web-based
Techniques: Activation Assay, Infection, Generated, Software
Journal: Tuberculosis (Edinburgh, Scotland)
Article Title: Differential inflammasome activation signatures following intracellular infection of human macrophages with Mycobacterium bovis BCG or Trypanosoma cruzi
doi: 10.1016/j.tube.2016.09.026
Figure Lengend Snippet: Human THP-1 cells were differentiated into macrophages by activation with PMA (50 ng/ml), rested, and infected with 3 M.O.I. of either M. bovis BCG or T. cruzi for 3 and 18 h. Transcripts were quantified using the RT Profiler Human Inflammasome PCR Array (SA Biosciences/Qiagen) and triplicate samples. Data generated was analyzed using the web-based PCR Array Data Analysis software for threshold cycle (Ct) value determinations and contrast analysis was performed using LimmaCt in the HT-qPCR software package to identify genes that were differentially expressed.
Article Snippet: Data generated was analyzed using the web-based
Techniques: Activation Assay, Infection, Generated, Software